Generator

Part:BBa_K201001

Designed by: francesca ceroni   Group: iGEM09_Bologna   (2009-10-11)

LacI repressed GFP generator with BBa_K201008

GFP reporter protein cloned downstream of the BBa_K201008 promoter (constitutive BBa_J23100 promoter plus O2 Lac operator). See the experience section for characterization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734


[edit]
Categories
Parameters
n/aLacI repressed GFP generator with BBa_K201008